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  • Featured Product
  • KD/KO Validated

TDP-43 (human specific) Monoclonal antibody, PBS Only (Capture/Detector)

TDP-43 (human specific) Monoclonal Antibody for WB, IP, FC, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, FC, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6H6E12

Cat no : 60019-2-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    WB analysis using 60019-2-Ig (same clone as 60019-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Standard curve of MP50030-1

    Standard curve of MP50030-1

    Standard curve of MP50030-1, TDP-43 Monoclonal Matched Antibody Pair - BSA and Azide free, measured by sandwich ELISA. Capture antibody: 66734-2-PBS. Detection antibody: 60019-2-PBS (Biotinylated). Standard: Ag13119. Range: 6.25-400 ng/mL.

  • Standard curve of MP80000-1

    Standard curve of MP80000-1

    Standard curve of MP80000-1, TDP-43 Hybrid Matched Antibody Pair - BSA and Azide free, measured by sandwich ELISA. Capture antibody: 60019-2-PBS. Detection antibody: 80001-1-PBS. Standard: Ag1833. Range: 78.1-5000 pg/mL.

  • Immunoprecipitation (IP) experiment of K-562 cells using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IP experiment of K-562 using 60019-2-Ig (same clone as 60019-2-PBS)

    IP result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug. This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human gliomas using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain(FTLD-U) tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human brain(FTLD-U) tissue using 60019-2-Ig (same clone as 60019-2-PBS)

    40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human pancreas cancer using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human pancreas cancer using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    FC experiment of MCF-7 using 60019-2-Ig (same clone as 60019-2-PBS)

    1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 (human specific) antibody (60019-2-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60019-2-PBS targets TDP-43 (human specific) as part of a matched antibody pair:

MP50030-1: 66734-2-PBS capture and 60019-2-PBS detection (validated in Sandwich ELISA)

MP80000-1: 60019-2-PBS capture and 80001-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.