TADA1L Recombinant antibody

TADA1L Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240637F7

Cat no : 83641-4-RR

Synonyms

STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764

---

Validation Data Gallery

• 

  WB analysis using 83641-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 83641-4-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 83641-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83641-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, human testis tissue, HepG2 cells, mouse testis tissue, rat testis tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83641-4-RR is TADA1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TADA1L fusion protein Ag14118
• Full Name: transcriptional adaptor 1 (HFI1 homolog, yeast)-like
• Calculated Molecular Weight: 335 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC015401
• Gene Symbol: TADA1L
• Gene ID (NCBI): 117143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.