TADA1L Recombinant antibody

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  WB analysis using 83641-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 83641-4-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 83641-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83641-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83641-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

TADA1L {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83641-4-RR

Clone No.240637F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764

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Tested Applications

• Positive WB detected in: MCF-7 cells, human testis tissue, HepG2 cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83641-4-RR targets TADA1L in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TADA1L fusion protein Ag14118
• Full Name: transcriptional adaptor 1 (HFI1 homolog, yeast)-like
• Calculated Molecular Weight: 335 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC015401
• Gene Symbol: TADA1L
• Gene ID (NCBI): 117143
• RRID: AB_3671252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96BN2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.