TACC3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TACC3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25697-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 25697-1-AP (same clone as 25697-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25697-1-AP (TACC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 25697-1-AP (same clone as 25697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 25697-1-AP (same clone as 25697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 25697-1-AP (same clone as 25697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 25697-1-AP (same clone as 25697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25697-1-AP (same clone as 25697-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TACC3 antibody (25697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

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Product Information

25697-1-PBS targets TACC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TACC3 fusion protein Ag22516
• Full Name: transforming, acidic coiled-coil containing protein 3
• Calculated Molecular Weight: 838 aa, 91 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC111771
• Gene Symbol: TACC3
• Gene ID (NCBI): 10460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. This antibody works well in WB, IHC and IF application.