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  • KD/KO Validated

TACC3 Polyclonal antibody

TACC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25697-1-AP

Synonyms

ERIC 1, ERIC1, TACC3



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25697-1-AP is TACC3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACC3 fusion protein Ag22516
Full Name transforming, acidic coiled-coil containing protein 3
Calculated Molecular Weight 838 aa, 91 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC111771
Gene Symbol TACC3
Gene ID (NCBI) 10460
RRIDAB_2880199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. This antibody works well in WB, IHC and IF application.

Protocols

Product Specific Protocols
WB protocol for TACC3 antibody 25697-1-APDownload protocol
IHC protocol for TACC3 antibody 25697-1-APDownload protocol
IF protocol for TACC3 antibody 25697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
humanWB,IHC

Oncotarget

TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.

Authors - Yong Du
  • KD Validated
humanWB

Cell Death Dis

PMID: 29358577

Authors - Zhi-Rui Lin
  • KD Validated
humanWB,IF

Vet Microbiol

Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import

Authors - Lulu Deng
  • KD Validated
human,mouseWB,IHC,IF

Cell Death Dis

Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer

Authors - Saimeng Shi
  • KD Validated