TACC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TACC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25697-1-AP

Synonyms

ERIC 1, ERIC1, TACC3

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Validation Data Gallery

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  WB analysis using 25697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25697-1-AP (TACC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25697-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TACC3 antibody (25697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

The immunogen of 25697-1-AP is TACC3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TACC3 fusion protein Ag22516
• Full Name: transforming, acidic coiled-coil containing protein 3
• Calculated Molecular Weight: 838 aa, 91 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC111771
• Gene Symbol: TACC3
• Gene ID (NCBI): 10460
• RRID: AB_2880199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. This antibody works well in WB, IHC and IF application.

Publications

Species	Application	Title
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB,IHC	Oncotarget; TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.; Authors - Yong Du ; KD Validated
human	WB	Cell Death Dis; PMID: 29358577; Authors - Zhi-Rui Lin ; KD Validated
human	WB,IF	Vet Microbiol; Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import; Authors - Lulu Deng ; KD Validated
human,mouse	WB,IHC,IF	Cell Death Dis; Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer; Authors - Saimeng Shi ; KD Validated