Filter: AllWBIHCIF/ICC

WB analysis using 60059-1-Ig (same clone as 60059-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 60059-1-Ig (same clone as 60059-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 60059-1-Ig (same clone as 60059-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
WB analysis of U-251 using 60059-1-Ig (same clone as 60059-1-PBS)
U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.
IF Staining of PC-12 using 60059-1-Ig (same clone as 60059-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (60059-1-Ig, Clone: 2B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

Syntaxin 6 Monoclonal antibody, PBS Only

Syntaxin 6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60059-1-PBS

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Syntaxin-6, STX6, 2B6G4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60059-1-PBS targets Syntaxin 6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Syntaxin 6 fusion protein Ag1284 Predict reactive species
Full Name	syntaxin 6
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC009944
Gene Symbol	Syntaxin 6
Gene ID (NCBI)	10228
RRID	AB_2196504
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O43752
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.