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Syntaxin 6 Monoclonal antibody

Syntaxin 6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6G4

Cat no : 60059-1-Ig

Synonyms

Syntaxin-6, STX6, 2B6G4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis using 60059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of Jurkat using 60059-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of SH-SY5Y using 60059-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of U-251 using 60059-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human brain using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human brain using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human prostate cancer using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human prostate cancer using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inC6 cells, SH-SY5Y cells, U-251 cells, Jurkat cells, Neuro-2a cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60059-1-Ig targets Syntaxin 6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284
Full Name syntaxin 6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2196504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 60059-1-IgDownload protocol
IHC protocol for Syntaxin 6 antibody 60059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy

Authors - Shouheng Jin
View Article