Syntaxin 1A / Syntaxin 1B Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D3B5

Cat no : 66437-1-PBS

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

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Validation Data Gallery

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  WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66437-1-Ig (same clone as 66437-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66437-1-PBS targets Syntaxin 1A / Syntaxin 1B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 1A / Syntaxin 1B fusion protein Ag10257
• Full Name: syntaxin 1B
• Calculated Molecular Weight: 288 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 55 kDa
• GenBank Accession Number: BC062298
• Gene Symbol: STX1B
• Gene ID (NCBI): 112755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).