Synaptotagmin-1 Monoclonal antibody, PBS Only

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H1H2

Cat no : 68043-1-PBS

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

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Validation Data Gallery

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  WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68043-1-PBS targets Synaptotagmin-1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synaptotagmin-1 fusion protein Ag6071
• Full Name: synaptotagmin I
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC058917
• Gene Symbol: SYT1
• Gene ID (NCBI): 6857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).