Synaptotagmin-1 Monoclonal antibody

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H1H2

Cat no : 68043-1-Ig

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

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Validation Data Gallery

• 

  WB analysis using 68043-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 68043-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 68043-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68043-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68043-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 68043-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 68043-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68043-1-Ig targets Synaptotagmin-1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit, Chicken
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synaptotagmin-1 fusion protein Ag6071
• Full Name: synaptotagmin I
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC058917
• Gene Symbol: Synaptotagmin-1
• Gene ID (NCBI): 6857
• RRID: AB_2918785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

Publications

Species	Application	Title
human	WB	F1000Res; A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry; Authors - Michael S Biddle