Stathmin 1 Recombinant antibody, PBS Only

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  WB analysis using 82559-1-RR (same clone as 82559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82559-1-RR (Stathmin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 82559-1-RR (same clone as 82559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82559-1-RR (same clone as 82559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 82559-1-RR (same clone as 82559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82559-1-RR (same clone as 82559-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 82559-1-RR (same clone as 82559-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82559-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82559-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82559-1-RR against Human Stathmin 1 were performed. The affinity constant is 7.57 nM.

Stathmin 1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 82559-1-PBS

Clone No.2H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18

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Product Information

82559-1-PBS targets Stathmin 1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Stathmin 1 fusion protein Ag1633
• Full Name: stathmin 1/oncoprotein 18
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC014353
• Gene Symbol: Stathmin 1
• Gene ID (NCBI): 3925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16949
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.