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Stathmin 1 Recombinant antibody

Stathmin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H6

Cat no : 82559-1-RR

Synonyms

STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Stathmin 1 Recombinant antibody (82559-1-RR)

    WB analysis using 82559-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82559-1-RR (Stathmin 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Stathmin 1 Recombinant antibody (82559-1-RR)

    IHC staining of rat brain using 82559-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Stathmin 1 Recombinant antibody (82559-1-RR)

    IHC staining of rat brain using 82559-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82559-1-RR (Stathmin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Stathmin 1 Recombinant antibody (82559-1-RR)

    IF Staining of HeLa using 82559-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Stathmin 1 Recombinant antibody (82559-1-RR)

    IF Staining of NIH/3T3 using 82559-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Stathmin 1 antibody (82559-1-RR, Clone: 2H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82559-1-RR

    Affinity and Kinetic Characterization of 82559-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82559-1-RR against Human Stathmin 1 were performed. The affinity constant is 7.57 nM.

Tested Applications

Positive WB detected inSH-SY5Y cells, Jurkat cells, PC-12 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82559-1-RR targets Stathmin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Stathmin 1 fusion protein Ag1633
Full Name stathmin 1/oncoprotein 18
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC014353
Gene Symbol Stathmin 1
Gene ID (NCBI) 3925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Protocols

Product Specific Protocols
WB protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
IHC protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
IF protocol for Stathmin 1 antibody 82559-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols