Stanniocalcin 2 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

Stanniocalcin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H1E7

Cat no : 60063-1-PBS

Synonyms

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

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Validation Data Gallery

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  WB analysis of A549 using 60063-1-Ig (same clone as 60063-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60063-1-Ig (same clone as 60063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 60063-1-Ig (same clone as 60063-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60063-1-Ig (same clone as 60063-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HCT 116 using 60063-1-Ig (same clone as 60063-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60063-1-Ig (same clone as 60063-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 60063-1-Ig (same clone as 60063-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60063-1-Ig (same clone as 60063-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 60063-1-Ig (same clone as 60063-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60063-1-Ig (same clone as 60063-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60063-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50715-1

  Cytometric bead array standard curve of MP50715-1, STC2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60063-1-PBS. Detection antibody: 60063-2-PBS. Standard:Ag0359. Range: 0.391-100 ng/mL.

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Product Information

60063-1-PBS targets Stanniocalcin 2 as part of a matched antibody pair:

MP50715-1: 60063-1-PBS capture and 60063-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Stanniocalcin 2 fusion protein Ag0359
• Full Name: stanniocalcin 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000658
• Gene Symbol: Stanniocalcin 2/STC2
• Gene ID (NCBI): 8614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.