Stanniocalcin 2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Stanniocalcin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

4H1E7

Cat no : 60063-1-Ig

Synonyms

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

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Validation Data Gallery

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  WB analysis of A549 using 60063-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60063-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 60063-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60063-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 60063-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 60063-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60063-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60063-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60063-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60063-1-Ig (Stanniocalcin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60063-1-Ig(Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50.

• 

  IHC staining of human stomach cancer using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 60063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60063-1-Ig (Stanniocalcin 2 antibody at dilution of 1:50.

• 

  IF Staining of HeLa using 60063-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60063-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Stanniocalcin 2 antibody (60063-1-Ig, Clone: 4H1E7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, hTERT-RPE1 cells, T-47D cells, HEK-293 cells, HT-29 cells, HCT 116 cells, HepG2 cells, PC-3 cells, pig brain tissue
• Positive IHC detected in: mouse kidney tissue, human breast cancer tissue, human kidney tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

60063-1-Ig targets Stanniocalcin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Stanniocalcin 2 fusion protein Ag0359
• Full Name: stanniocalcin 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000658
• Gene Symbol: Stanniocalcin 2/STC2
• Gene ID (NCBI): 8614
• RRID: AB_2197544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Publications

Species	Application	Title
human	IHC	J Hepatocell Carcinoma; The Oncogenic and Diagnostic Potential of Stanniocalcin 2 in Hepatocellular Carcinoma.; Authors - Zhixian WuJin
human	IHC, WB	Biochem Biophys Res Commun; The novel prognostic risk factor STC2 can regulate the occurrence and progression of osteosarcoma via the glycolytic pathway.; Authors - Bo Yu ; KD Validated
human	WB	Biomed Res Int; STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.; Authors - Chen Lin ; KD Validated
human	WB	Oncotarget; STC2 promotes head and neck squamous cell carcinoma metastasis through modulating the PI3K/AKT/Snail signaling.; Authors - Shuwen Yang ; KD Validated
mouse	WB	Neuroscience; Profiling the Gene Expression and DNA Methylation in the Mouse Brain after Ischemic Preconditioning.; Authors - Menghua Cai
human	IHC	BMC Med Genomics; Osteosarcoma transcriptome data exploration reveals STC2 as a novel risk indicator in disease progression; Authors - Ziyue Wang