Spermidine synthase Monoclonal antibody, PBS Only

• 

  WB analysis using 68548-1-Ig (same clone as 68548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68548-1-Ig (Spermidine synthase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 68548-1-Ig (same clone as 68548-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Spermidine synthase antibody (68548-1-Ig, Clone: 2F4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

Spermidine synthase Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68548-1-PBS

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1

---

Product Information

68548-1-PBS targets Spermidine synthase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Spermidine synthase fusion protein Ag33919
• Full Name: spermidine synthase
• Calculated Molecular Weight: 302 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000309
• Gene Symbol: Spermidine synthase
• Gene ID (NCBI): 6723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19623
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).