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Spermidine synthase Monoclonal antibody

Spermidine synthase Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4E10

Cat no : 68548-1-Ig

Synonyms

PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    WB analysis using 68548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68548-1-Ig (Spermidine synthase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    IF Staining of RAW 264.7 using 68548-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Spermidine synthase antibody (68548-1-Ig, Clone: 2F4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68548-1-Ig targets Spermidine synthase in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Spermidine synthase fusion protein Ag33919
Full Name spermidine synthase
Calculated Molecular Weight 302 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000309
Gene Symbol Spermidine synthase
Gene ID (NCBI) 6723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Protocols

Product Specific Protocols
WB protocol for Spermidine synthase antibody 68548-1-IgDownload protocol
IF protocol for Spermidine synthase antibody 68548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols