Sestrin 2 Recombinant antibody, PBS Only (Detector)

Sestrin 2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240034H3

Cat no : 83202-3-PBS

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

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Validation Data Gallery

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  WB analysis using 83202-3-RR (same clone as 83202-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 83202-3-RR (same clone as 83202-3-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00278-1

  Cytometric bead array standard curve ofMP00278-1, Sestrin2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83202-2-PBS. Detection antibody: 83202-3-PBS. Standard: Ag1247. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83202-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

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Product Information

83202-3-PBS targets Sestrin 2 as part of a matched antibody pair:

MP00278-1: 83202-2-PBS capture and 83202-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Sestrin 2 fusion protein Ag1247
• Full Name: sestrin 2
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: BC013304
• Gene Symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.