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Sestrin 2 Recombinant antibody

Sestrin 2 Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

240034H3

Cat no : 83202-3-RR

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Sestrin 2 Recombinant antibody (83202-3-RR)

    WB analysis using 83202-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using Sestrin 2 Recombinant antibody (83202-3-RR)

    FC experiment of HEK-293 using 83202-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83202-3-RR

    Affinity and Kinetic Characterization of 83202-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83202-3-RR targets Sestrin 2 in WB, ELISA, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Sestrin 2 fusion protein Ag1247
Full Name sestrin 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
FC protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols