Sestrin 2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Sestrin 2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 66297-1-PBS

Synonyms

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

---

Validation Data Gallery

• 

  WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

  WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66297-1-Ig (same clone as 66297-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66297-1-Ig (same clone as 66297-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66297-1-Ig (same clone as 66297-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66297-1-Ig (same clone as 66297-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66297-1-PBS targets Sestrin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Sestrin 2 fusion protein Ag20638
• Full Name: sestrin 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC013304
• Gene Symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).