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SerpinB3/SerpinB4 Polyclonal antibody, PBS Only

SerpinB3/SerpinB4 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11428-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SerpinB3/SerpinB4 Polyclonal antibody (11428-1-AP)

    WB analysis using 11428-1-AP (same clone as 11428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SerpinB3/SerpinB4 Polyclonal antibody (11428-1-AP)

    WB analysis of COLO 320 using 11428-1-AP (same clone as 11428-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11428-1-PBS in a different storage buffer formulation.

Product Information

11428-1-PBS targets SerpinB3/SerpinB4 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SerpinB3/SerpinB4 fusion protein Ag1964
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 4
Calculated Molecular Weight 390 aa, 45 kDa
Observed Molecular Weight42-45 kDa
GenBank Accession NumberBC017401
Gene Symbol SERPINB4
Gene ID (NCBI) 6318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.