SerpinB3/SerpinB4 Polyclonal antibody

SerpinB3/SerpinB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11428-1-AP

Synonyms

LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4

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Validation Data Gallery

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  WB analysis using 11428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11428-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, COLO 320 cells, human saliva

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11428-1-AP targets SerpinB3/SerpinB4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SerpinB3/SerpinB4 fusion protein Ag1964
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 4
• Calculated Molecular Weight: 390 aa, 45 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC017401
• Gene Symbol: SERPINB4
• Gene ID (NCBI): 6318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.