CoraLite® Plus 488-conjugated Serpin C1/Antithrombin III Monoclonal antibody

Serpin C1/Antithrombin III Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B2E5

Cat no : CL488-66052

Synonyms

Antithrombin, Antithrombin III, AT3, ATIII, HEPARIN COFACTOR I, Serpin C1, SERPINC1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66052

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Serpin C1/Antithrombin III antibody (CL488-66052, Clone: 2B2E5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66052 targets Serpin C1/Antithrombin III in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Serpin C1/Antithrombin III fusion protein Ag9880
• Full Name: serpin peptidase inhibitor, clade C (antithrombin), member 1
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC022309
• Gene Symbol: Serpin C1/Antithrombin III
• Gene ID (NCBI): 462
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.