SYTL4 Recombinant antibody

SYTL4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240556E2

Cat no : 83564-5-RR

Synonyms

synaptotagmin like 4, SLP4, Granuphilin, Exophilin-2, Exophilin 2

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Validation Data Gallery

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  WB analysis using 83564-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83564-5-RR (SYTL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83564-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83564-5-RR against Human SYTL4 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83564-5-RR is SYTL4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SYTL4 fusion protein Ag2772
• Full Name: synaptotagmin-like 4
• Calculated Molecular Weight: 671 aa, 76 kDa
• Observed Molecular Weight: 74-76 kDa
• GenBank Accession Number: BC014913
• Gene Symbol: SYTL4
• Gene ID (NCBI): 94121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)