CoraLite®594-conjugated SYNPO Monoclonal antibody

SYNPO Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

1E1B1

Cat no : CL594-67339

Synonyms

KIAA1029, synaptopodin, SYNPO

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67339

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SYNPO antibody (CL594-67339, Clone: 1E1B1 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-67339 targets SYNPO in IF-P applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SYNPO fusion protein Ag15089
• Full Name: synaptopodin
• Calculated Molecular Weight: 929 aa, 99 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC146665
• Gene Symbol: SYNPO
• Gene ID (NCBI): 11346
• RRID: AB_2920116
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publications

Species	Application	Title
	IF	J Am Soc Nephrol; Podocyte Geranylgeranyl Transferase Type-I Is Essential for Maintenance of the Glomerular Filtration Barrier; Authors - Roberto Boi