SYNGR1 Monoclonal antibody, PBS Only

SYNGR1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H2H1

Cat no : 68176-1-PBS

Synonyms

synaptogyrin 1, SYNGR1

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Validation Data Gallery

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  WB analysis using 68176-1-Ig (same clone as 68176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68176-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse retina using 68176-1-Ig (same clone as 68176-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68176-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68176-1-Ig (same clone as 68176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68176-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68176-1-Ig (same clone as 68176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68176-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68176-1-Ig (same clone as 68176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (68176-1-Ig, Clone: 2H2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68176-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68176-1-PBS targets SYNGR1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SYNGR1 fusion protein Ag31309
• Full Name: synaptogyrin 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000731
• Gene Symbol: SYNGR1
• Gene ID (NCBI): 9145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.