SYNGR1 Monoclonal antibody

SYNGR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H2H1

Cat no : 68176-1-Ig

Synonyms

synaptogyrin 1, SYNGR1

---

Validation Data Gallery

• 

  WB analysis using 68176-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of mouse retina using 68176-1-Ig

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 68176-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 68176-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 68176-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (68176-1-Ig, Clone: 2H2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse retina tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68176-1-Ig targets SYNGR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SYNGR1 fusion protein Ag31309
• Full Name: synaptogyrin 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000731
• Gene Symbol: SYNGR1
• Gene ID (NCBI): 9145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.