SYN1-Specific Recombinant antibody

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  WB analysis using 85360-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85360-1-RR (SYN1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse retina using 85360-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using SYN1-Specific antibody (85360-1-RR, Clone: 242415H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYN1-Specific {ptg:Brand} Recombinant Antibody for WB, IF-P, ELISA

Cat No. 85360-1-RR

Clone No.242415H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

SYN1, Synapsin I, 242415H5, Brain protein 4.1, SYN1a

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Tested Applications

• Positive WB detected in: IMR-32 cells, SH-SY5Y cells, SK-N-SH cells
• Positive IF-P detected in: mouse retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85360-1-RR targets SYN1-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: synapsin I
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_006950
• Gene Symbol: SYN1
• Gene ID (NCBI): 6853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17600
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).