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CoraLite® Plus 488-conjugated SYK Monoclonal antibody

SYK Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C4A12

Cat no : CL488-66721

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK



Tested Applications

Positive WB detected inDaudi cells, Ramos cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66721 targets SYK in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYK fusion protein Ag17654
Full Name spleen tyrosine kinase
Calculated Molecular Weight 635 aa, 72 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 SYK antibody CL488-66721Download protocol
Standard Protocols
Click here to view our Standard Protocols