Phospho-SYK (Tyr323) Recombinant antibody, PBS Only

Phospho-SYK (Tyr323) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H24

Cat no : 83331-1-PBS

Synonyms

SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

---

Validation Data Gallery

• 

  WB analysis using 83331-1-RR (same clone as 83331-1-PBS)

  Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Ramos using 83331-1-RR (same clone as 83331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

---

Product Information

83331-1-PBS targets Phospho-SYK (Tyr323) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 72 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.