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Phospho-SYK (Tyr323) Recombinant antibody

Phospho-SYK (Tyr323) Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H24

Cat no : 83331-1-RR

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, Phospho-SYK (Tyr323), spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using Phospho-SYK (Tyr323) Recombinant antibody (83331-1-RR)

    WB analysis of Ramos using 83331-1-RR

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using Phospho-SYK (Tyr323) Recombinant antibody (83331-1-RR)

    IF Staining of Ramos using 83331-1-RR

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inpervanadate treated Ramos cells
Positive IF detected inpervanadate treated Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name spleen tyrosine kinase
Calculated Molecular Weight 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

Protocols

Product Specific Protocols
WB protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
IF protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols