SYCP1 Recombinant antibody

SYCP1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240033E3

Cat no : 83382-1-RR

Synonyms

SYCP 1, SCP-1, SCP1, SCP 1, CT8

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Validation Data Gallery

• 

  WB analysis of mouse testis using 83382-1-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 83382-1-RR (SYCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of MCF-7 using 83382-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Sycp1 Recombinant Antibody (83382-1-RR, Clone:240033E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83382-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83382-1-RR against Human SYCP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83382-1-RR is SYCP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SYCP1 fusion protein Ag16005
• Full Name: synaptonemal complex protein 1
• Calculated Molecular Weight: 976 aa, 114 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC126266
• Gene Symbol: SYCP1
• Gene ID (NCBI): 6847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.