SUSD2 Polyclonal antibody

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  WB analysis using 30711-1-AP

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 30711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30711-1-AP (SUSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SUSD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30711-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

sushi domain containing 2, BK65A6.2

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Tested Applications

• Positive WB detected in: SK-BR-3 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30711-1-AP targets SUSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUSD2 fusion protein Ag33630
• Full Name: sushi domain containing 2
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 60 kDa, 90 kDa, 110 kDa
• GenBank Accession Number: NM_019601
• Gene Symbol: SUSD2
• Gene ID (NCBI): 56241
• RRID: AB_3669753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGT4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).