SURVIVIN Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SURVIVIN Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B9H7

Cat no : 66495-1-PBS

Synonyms

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

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Validation Data Gallery

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  WB analysis using 66495-1-Ig (same clone as 66495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66495-1-Ig (same clone as 66495-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66495-1-Ig (same clone as 66495-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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Product Information

66495-1-PBS targets SURVIVIN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SURVIVIN fusion protein Ag20958
• Full Name: baculoviral IAP repeat-containing 5
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC008718
• Gene Symbol: Survivin
• Gene ID (NCBI): 332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.