SURF1 Recombinant antibody, PBS Only

SURF1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230210D5

Cat no : 83052-7-PBS

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210D5

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Validation Data Gallery

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  IP experiment of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  IP result of anti-SURF1 (IP:83052-7-RR, 4ug; Detection:83052-7-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83052-7-RR (same clone as 83052-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-7-RR, Clone:230210D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83052-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83052-7-RR against Human SURF1 were performed. The affinity constant is below 1 pM.

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Product Information

83052-7-PBS targets SURF1 in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SURF1 fusion protein Ag3959
• Full Name: surfeit 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-31 kDa
• GenBank Accession Number: BC028314
• Gene Symbol: SURF1
• Gene ID (NCBI): 6834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.