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SURF1 Recombinant antibody

SURF1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230210D5

Cat no : 83052-7-RR

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210D5

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using SURF1 Recombinant antibody (83052-7-RR)

    IP experiment of HeLa using 83052-7-RR

    IP result of anti-SURF1 (IP:83052-7-RR, 4ug; Detection:83052-7-RR 1:2000) with HeLa cells lysate 1050 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SURF1 Recombinant antibody (83052-7-RR)

    IHC staining of human liver cancer using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SURF1 Recombinant antibody (83052-7-RR)

    IHC staining of human liver cancer using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SURF1 Recombinant antibody (83052-7-RR)

    IHC staining of mouse heart using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SURF1 Recombinant antibody (83052-7-RR)

    IHC staining of mouse heart using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SURF1 Recombinant antibody (83052-7-RR)

    IF Staining of HeLa using 83052-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SURF1 Recombinant antibody (83052-7-RR)

    IF Staining of HeLa using 83052-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using SURF1 Recombinant antibody (83052-7-RR)

    FC experiment of HepG2 using 83052-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-7-RR, Clone:230210D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83052-7-RR

    Affinity and Kinetic Characterization of 83052-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-7-RR against Human SURF1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83052-7-RR targets SURF1 in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SURF1 fusion protein Ag3959
Full Name surfeit 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SURF1 antibody 83052-7-RRDownload protocol
IF protocol for SURF1 antibody 83052-7-RRDownload protocol
IP protocol for SURF1 antibody 83052-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols