SURF1 Recombinant antibody, PBS Only (Capture)

SURF1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230210D1

Cat no : 83052-2-PBS

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210D1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00034-4

  Sandwich ELISA standard curve of MP00034-4, Human SURF1 Recombinant Matched Antibody Pair - PBS only. 83052-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3959. 83052-8-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

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  Sample test of MP00034-4

  Hela and MCF-7 cell lysates were measured. The human SURF1 concentration of detected samples was determined to be 3,709.4 pg/mL (based on a 6.4 mg/mL extract load) in Hela cell lyaste and 2,335.0 pg/mL (based on a 4.3 mg/mL extract load) in MCF-7 cell lysate.

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  Cytometric bead array standard curve of MP00034-3

  Cytometric bead array standard curve of MP00034-3, SURF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83052-2-PBS. Detection antibody: 83052-5-PBS. Standard: Ag3959. Range: 0.625-20 ng/mL.

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Product Information

83052-2-PBS targets SURF1 as part of a matched antibody pair:

MP00034-3: 83052-2-PBS capture and 83052-5-PBS detection (validated in Cytometric bead array)

MP00034-4: 83052-2-PBS capture and 83052-8-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SURF1 fusion protein Ag3959
• Full Name: surfeit 1
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC028314
• Gene Symbol: SURF1
• Gene ID (NCBI): 6834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.