SUMO2/3 Recombinant antibody

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  IF Staining of HepG2 using 84017-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84017-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84017-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO2/3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  Affinity and Kinetic Characterization of 84017-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84017-5-RR against Human SUMO2/3 were performed. The affinity constant is 6.81 nM.

SUMO2/3 Recombinant Antibody for IF/ICC, ELISA

Cat No. 84017-5-RR

Clone No.241156E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

SUMO3, SUMO 2, SMT3H1, SMT3A, SMT3 homolog 1

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84017-5-RR targets SUMO2/3 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SUMO2/3 fusion protein Ag1135
• Full Name: SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: BC008420
• Gene Symbol: SUMO3
• Gene ID (NCBI): 6612
• RRID: AB_3671586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55854
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.