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SUMO2/3 Recombinant antibody, PBS Only

SUMO2/3 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241156E1

Cat no : 84017-5-PBS

Synonyms

SUMO3, SUMO 2, SMT3H1, SMT3A, SMT3 homolog 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUMO2/3 Recombinant antibody (84017-5-RR)

    IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SUMO2/3 Recombinant antibody (84017-5-RR)

    IF Staining of A431 using 84017-5-RR (same clone as 84017-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUMO2/3 Recombinant antibody (84017-5-RR)

    IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO2/3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84017-5-RR

    Affinity and Kinetic Characterization of 84017-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84017-5-RR against Human SUMO2/3 were performed. The affinity constant is 6.81 nM.

Product Information

84017-5-PBS targets SUMO2/3 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1135
Full Name SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Calculated Molecular Weight 12 kDa
GenBank Accession NumberBC008420
Gene Symbol SUMO3
Gene ID (NCBI) 6612
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.