CoraLite® Plus 488-conjugated SUMO1 Polyclonal antibody

SUMO1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10329

Synonyms

Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1

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Validation Data Gallery

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  IF Staining of A549 using CL488-10329

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 SUMO1 antibody (CL488-10329) at dilution of 1:400.

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  FC experiment of A549 using CL488-10329

  1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SUMO1 (CL488-10329) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10329 targets SUMO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUMO1 fusion protein Ag0414
• Full Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10-12 kDa, 80-90 kDa
• GenBank Accession Number: BC006462
• Gene Symbol: SUMO1
• Gene ID (NCBI): 7341
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).