CoraLite® Plus 488-conjugated SULT4A1 Monoclonal antibody

SULT4A1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9F6

Cat no : CL488-66106

Synonyms

NST, hBR-STL-1, hBR-STL, EC:2.8.2.-, Brain sulfotransferase-like protein

---

Validation Data Gallery

• 

  IF Staining of mouse brain using CL488-66106

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 SULT4A1 antibody (CL488-66106, Clone: 1B9F6 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using CL488-66106

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Sult4a1 Monoclonal Antibody (CL488-66106, Clone:1B9F6)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66106 targets SULT4A1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SULT4A1 fusion protein Ag17962
• Full Name: sulfotransferase family 4A, member 1
• Calculated Molecular Weight: 284 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC022459
• Gene Symbol: SULT4A1
• Gene ID (NCBI): 25830
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.