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CoraLite® Plus 488-conjugated SUFU Polyclonal antibody

SUFU Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26759

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

UNQ650/PRO1280, Suppressor of fused homolog, SUFUH, PRO1280

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26759 targets SUFU in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUFU fusion protein Ag25200 Predict reactive species
Full Name suppressor of fused homolog (Drosophila)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC013291
Gene Symbol SUFU
Gene ID (NCBI) 51684
RRIDAB_3084100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMX1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
FC protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
Standard Protocols
Click here to view our Standard Protocols
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