SUCLG1 Polyclonal antibody, PBS Only

SUCLG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14923-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14923-1-AP (same clone as 14923-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 14923-1-AP (same clone as 14923-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

  IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14923-1-AP (same clone as 14923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14923-1-AP (SUCLG1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

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Product Information

14923-1-PBS targets SUCLG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUCLG1 fusion protein Ag6481
• Full Name: succinate-CoA ligase, alpha subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000504
• Gene Symbol: SUCLG1
• Gene ID (NCBI): 8802
• RRID: AB_2197177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).