SUCLG1 Polyclonal antibody

SUCLG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14923-1-AP

Synonyms

G ALPHA, SCS alpha, SUCLA1, SUCLG1

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Validation Data Gallery

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  WB analysis using 14923-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14923-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14923-1-AP

  IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug.

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  IHC staining of human liver cancer using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14923-1-AP (SUCLG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse kidney using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, rat liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

14923-1-AP targets SUCLG1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUCLG1 fusion protein Ag6481
• Full Name: succinate-CoA ligase, alpha subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000504
• Gene Symbol: SUCLG1
• Gene ID (NCBI): 8802
• RRID: AB_2197177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely
mouse	WB	Nat Commun; A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages; Authors - Pengkai Sun
mouse	WB,IP	J Mol Cell Cardiol; Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.; Authors - Xin Liu
human	IHC	Clin Cancer Res; Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics; Authors - Xiaoqi Peng
mouse	WB	Environ Toxicol; Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse; Authors - Yue-Cen Liu