STX3 Monoclonal antibody, PBS Only

STX3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9C7

Cat no : 66760-1-PBS

Synonyms

STX3, STX3A, syntaxin 3

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Validation Data Gallery

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  WB analysis using 66760-1-Ig (same clone as 66760-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66760-1-Ig (same clone as 66760-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66760-1-Ig (same clone as 66760-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66760-1-Ig (same clone as 66760-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66760-1-Ig (same clone as 66760-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66760-1-Ig (same clone as 66760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66760-1-Ig (same clone as 66760-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66760-1-PBS targets STX3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STX3 fusion protein Ag7805
• Full Name: syntaxin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC007405
• Gene Symbol: STX3
• Gene ID (NCBI): 6809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).