STX3 Monoclonal antibody

STX3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B9C7

Cat no : 66760-1-Ig

Synonyms

STX3, STX3A, syntaxin 3

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Validation Data Gallery

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  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66760-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66760-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66760-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HSC-T6 cells, rat brain tissue, SH-SY5Y cells, NIH/3T3 cell, RAW 264.7 cell, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66760-1-Ig targets STX3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STX3 fusion protein Ag7805
• Full Name: syntaxin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC007405
• Gene Symbol: STX3
• Gene ID (NCBI): 6809
• RRID: AB_2882106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

Species	Application	Title
human	IHC,WB	Cancer Med; Increased STX3 transcript and protein levels were associated with poor prognosis in two independent cohorts of esophageal squamous cell carcinoma patients; Authors - Takahiro Shinozuka