CoraLite® Plus 488-conjugated STK39 Recombinant antibody

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  IF Staining of mouse brain using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200.

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  FC experiment of Daudi using CL488-83081-2

  1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Stk39 Recombinant Antibody (CL488-83081-2, Clone:230382G9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

STK39 Recombinant Antibody for IF/ICC, IF-P

Cat No. CL488-83081-2

Clone No.230382G9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IF-P

Synonyms

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: Daudi cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83081-2 targets STK39 in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STK39 fusion protein Ag34503
• Full Name: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC166614
• Gene Symbol: STK39
• Gene ID (NCBI): 27347
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UEW8
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).