STK32A Monoclonal antibody, PBS Only (Capture)

STK32A Monoclonal Antibody for Cytometric bead array, Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1H2A6

Cat no : 67528-1-PBS

Synonyms

Yet another novel kinase 1, YANK1, Serine/threonine-protein kinase 32A, serine/threonine kinase 32A, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis of A549 using 67528-1-Ig (same clone as 67528-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67528-1-Ig (same clone as 67528-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67528-1-Ig (same clone as 67528-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 67528-1-Ig (same clone as 67528-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67528-1-Ig (same clone as 67528-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67528-1-Ig (same clone as 67528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67528-1-Ig (same clone as 67528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67528-1-Ig (same clone as 67528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67528-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50267-1

  Cytometric bead array standard curve of MP50267-1, STK32A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67528-1-PBS. Detection antibody: 67528-2-PBS. Standard:Ag10942. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP50267-2

  Cytometric bead array standard curve of MP50267-2, STK32A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67528-1-PBS. Detection antibody: 67528-3-PBS. Standard:Ag10942. Range: 1.563-200 ng/mL.

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Product Information

67528-1-PBS targets STK32A as part of a matched antibody pair:

MP50267-1: 67528-1-PBS capture and 67528-2-PBS detection (validated in Cytometric bead array)

MP50267-2: 67528-1-PBS capture and 67528-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STK32A fusion protein Ag10942
• Full Name: serine/threonine kinase 32A
• Calculated Molecular Weight: 396aa,46 kDa; 166aa,20 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC021666
• Gene Symbol: STK32A
• Gene ID (NCBI): 202374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.