Filter: AllWBIHC

WB analysis of A549 using 67528-1-Ig
A549 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67528-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 67528-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 67528-1-Ig
T-47D cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of JAR using 67528-1-Ig
JAR cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 67528-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67528-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67528-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STK32A Monoclonal antibody

Name: STK32A Monoclonal antibody
Brand: Proteintech
SKU: 67528-1-Ig

STK32A Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67528-1-Ig

Clone No.1H2A6

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

serine/threonine kinase 32A, STK32A, YANK1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, MCF-7 cells, T-47D cells, JAR cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67528-1-Ig targets STK32A in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	STK32A fusion protein Ag10942 Predict reactive species
Full Name	serine/threonine kinase 32A
Calculated Molecular Weight	396aa,46 kDa; 166aa,20 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC021666
Gene Symbol	STK32A
Gene ID (NCBI)	202374
RRID	AB_2882747
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8WU08
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.