STK24 Monoclonal antibody

STK24 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B4C1

Cat no : 68522-1-Ig

Synonyms

Serine/threonine-protein kinase 24 36 kDa subunit, Serine/threonine-protein kinase 24 12 kDa subunit, MST3, Mammalian STE20-like protein kinase 3, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 68522-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68522-1-Ig (STK24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 68522-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using STK24 antibody (68522-1-Ig, Clone: 2B4C1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68522-1-Ig targets STK24 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STK24 fusion protein Ag31526
• Full Name: serine/threonine kinase 24 (STE20 homolog, yeast)
• Calculated Molecular Weight: 49KD
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_003576
• Gene Symbol: STK24
• Gene ID (NCBI): 8428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).