STIM1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

STIM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3A3

Cat no : 66189-1-PBS

Synonyms

STIM1

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Validation Data Gallery

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  WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66189-1-Ig (same clone as 66189-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66189-1-PBS targets STIM1 in WB, IHC, Indirect ELISA applications and shows reactivity with rat, human, mouse samples.

• Tested Reactivity: rat, human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STIM1 fusion protein Ag17914
• Full Name: stromal interaction molecule 1
• Calculated Molecular Weight: 685 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC021300
• Gene Symbol: STIM1
• Gene ID (NCBI): 6786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.