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- KD/KO Validated
STIM1 Monoclonal antibody
STIM1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D3A3
Cat no : 66189-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
66189-1-Ig targets STIM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | STIM1 fusion protein Ag17914 |
| Full Name | stromal interaction molecule 1 |
| Calculated Molecular Weight | 685 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC021300 |
| Gene Symbol | STIM1 |
| Gene ID (NCBI) | 6786 |
| RRID | AB_2881584 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STIM1 antibody 66189-1-Ig | Download protocol |
| IHC protocol for STIM1 antibody 66189-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Biomed Pharmacother Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.
| ||
J Transl Med Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X | ||
Neurotoxicology STIM1 Mediates Methamphetamine-Induced Neuronal Autophagy and Apoptosis
|
